Research Interests
Lab Members
Current Open Positions
Leukocyte Adhesion Tutorial


2000 - PRESENT

To view all of Dr. Ley's publications via PubMed, please click here.

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Original Articles in Peer-Reviewed Journals

Publication Year
2010 Malpighi Award Lecture Ley, K. 2010
Rolling on E- or P-selectin induces the extended, but not high affinity conformation of LFA-1 in neutrophils. Kuwano, Y., Spelten, O., Zhang, H., Ley, K., Zarbock, A. Blood 2010
The transmembrane domains of L-selectin and CD44 regulate receptor cell surface positioning and leukocyte adhesion under flow. Buscher, K., Riese, S.B., Shakibaei, M., Reich, C., Dernedde, J., Tauber, R., Ley, K. Journal of Biological Chemistry 2010
Mycophenolic acid suppresses granulopoiesis by inhibition of interleukin 17 production. von Vietinghoff, S., Ouyang, H., Ley, K. Kidney International 2 2010
CXCL4 induces a unique transcriptome in monocyte-derived macrophages. Gleissner, G.A., Shaked, I., Little, K.M., Ley, K. Journal of Immunology 2010
Blockade of IL-17A results in reduced atherosclerosis in Apoe-deficient mice. Smith, E., Prasad, K.R., Butcher, M., Dobrian, A., Kolls, J.K., Ley, K., Galkina, E. Circulation 2010
Tyrosine kinase Btk regulates E-selectin-mediated integrin activation and neutrophil recruitment by controlling phospholipase C (PLC) ?2 and PI3K? pathways. Mueller, H., Stadtmann, A. Zhang, H., Van Aken, H., Hirsch, E. Wang, D., Ley, K., Zarbock A. Blood 2010
Identification of novel downstream targets of platelet glycoprotein VI activation by differential proteome analysis – implications for thrombus formation. Schulz, C., Leuschen, N.V., Fröhlich, T., Lorenz, M., Pfeiler, S., Gleissner, C.A., Kremmer, E., Kessler, M., Khandoga, A.G., Engelmann, B., Ley, K., Massberg, S., Arnold, G.J. Blood. 2010
CXCL4 downregulates the atheroprotective hemoglobin receptor CD163 in human macrophages. Gleissner, C.A., Shaked, I., Erbel, C., Böckler, D., Katus, H.A., Ley, K. Circulation Research 2010
ReSASC: A Resampling-Based Algorithm to Determine Differential Protein Expression from Spectral Count Data. Little, K.M., Lee, J.K., Ley, K. Proteomics 2010
PI3K ? required for LPS-induced transepithelial neutrophil trafficking in the lung. Reutershan, J., Saprito, M.S., Wu, D., Rückle, T., Ley, K. European Respiratory Journal. 2009
T-lineage cells require the thymus, but not V(D)J recombination to produce IL-17A and regulate granulopoiesis in vivo. Smith, E., von Vietinghoff, S., Stark, M.A., Zarbock, A., Sanders, J.M.., Duley, A., Rivera-Nieves, J., Bender, T.P., Ley, K. Journal of Immunology 2009
Improved survival and reduced vascular permeability by eliminating or blocking 12/15-lipoxygenase in mouse models of acute lung injury (ALI). Zarbock, A., DiStasi, M.R., Smith, E., Sanders, J.M., Kronke, G, Harry, B., von Vietinghoff, S., Buscher, K., Nadler, J.L., Ley, K. J. Immunol. 2009
Dual targeting improves microbubble contrast agent adhesion to VCAM-1 and P-selectin under flow. Ferrante, E.A., Pickard, J.E., Ryckak, J.J., Klibanov, A.S., Ley, K. Journal of Controlled Release 2009
Micro-PTV measurement of the fluid shear stress acting on adherent leukocytes in vivo. Pickard, J.E., Ley, K. Biophys. J. 2009
DARC on red blood cells limits lung injury by balancing compartmental distribution of CXC chemokines. Reutershan, J., Harry, B., Chang, D., Bagby, G.J., Ley, K. European Journal of Immunology 2009
Interleukin 17A controls Interleukin 17F production and maintains blood neutrophil counts in mice. von Vietinghoff, S., Ley, K. J. Immunol. 2009
Dynamics of Microvillus Extension and Tether Formation in Rolling Leukocytes. Pospieszalska, Ley, K. Cellular and Molecular Bioengineering 2009
Role of β7 integrins in intestinal lymphocyte homing and retention. Gorfu, G., Rivera-Nieves, J., Ley, K. Current Molecular Medicine 2009
Glycans in immune cell trafficking. Sperandio, M., Ley, K. Immunological Reviews 2009
The role of platelets in acute lung injury (ALI). Zarbock, A., Ley, K. Frontiers in Bioscience 2009
CD40 ligand promotes Mac-1 expression, leukocyte recruitment, and neointima formation after vascular injury Li G, Sanders JM, Bevard MH, Sun Z, Chumley JW, Galkina EV, Ley K, Sarembock IJ Am J Pathol 2008
PSGL-1 engagement by E-selectin signals through Src kinase Fgr and ITAM-adapters DAP12 and FcRγ to induce slow leukocyte rolling Zarbock, A., Abram, C.L., Hundt, M., Altman, A., Lowell, C.A., Ley, K. J. Exp. Med 2008
Transcriptional Regulation of mouse L-Selectin Dang, X., Raffler, N.A., K. Ley Biochim Biophys Acta 2009
Event tracking model of adhesion identifies load-bearing bonds in leukocyte rolling Pospieszalska, M.K., Zarbock, A., Pickard, J.E., Ley, K. Microcirculation 2009
Mechanisms and consequences of neutrophil interaction with the endothelium Zarbock, A., Ley, K. Biological Perspectives Series, Am. J. Pathol 2008
Plasma-derived microparticles for biomarker discovery Smalley, D.M, Ley, K. Clinical Laboratory 2008
Cells on the run: shear-regulated integrin activation in leukocyte rolling and arrest on endothelial cells Alon, R., Ley, K. Current Opinion in Cell Biology 2008
Neutrophil adhesion and activation under flow Zarbock, A., Ley, K. Microcirculation 2008
Leukocyte adhesion molecules in animal models of Inflammatory Bowel Disease Rivera-Nieves, J., Gorfu, G., Ley, K. Inflammatory Bowel Diseases 2008
Homeostatic regulation of blood neutrophil counts Vietinghoff, S., Ley, K. J. Immunol. 2008
Immune and inflammatory mechanisms of atherosclerosis Galkina, E., Ley, K. Ann. Rev. Immunology 2009
Monocyte-Endothelial Cell Interactions in the Development of Atherosclerosis Mestas, J., Ley, K. Trends Cardiovasc Med 2009
Dances with leukocytes: how tetraspanin-enriched microdomains assemble to form endothelial adhesive platforms Ley, K. and Zhang, H. J.Cell Biol 2008
Event tracking model of adhesion identifies load-bearing bonds in leukocyte rolling 13. Pospieszalska, M.K., Zarbock, A., Pickard, J.E., Ley, K. Microcirculation 2009
Endothelial Cell PECAM-1 Promotes Atherosclerotic Lesions in Areas of Disturbed Flow in ApoE-Deficient Mice Brian L. Harry, John M. Sanders, Ryan E. Feaver, Melissa Lansey, Tracy L. Deem, Alexander Zarbock, Anthony C. Bruce, Andrew W. Pryor, Bradley D. Gelfand, Brett R. Blackman, Martin A. Schwartz, Klaus Ley ATVB 2008
Platelet Chemokines in Vascular Disease Christian A. Gleissner, Philipp von Hundelshausen and Klaus Ley ATVB 2008
PECAM-1-dependent endothelial shear stress response promotes atherosclerotic lesions in areas of disturbed flow. Harry, B.L., Lansey, M., Sanders, J.M., Bruce, A., Pryor, A.W., Gelfand, B.D., Blackman, B.R., Schwartz, M.A., Ley, K. ATVB PMID 2008
Therapeutic inhibition of CXCR2 by Reparixin attenuates acute lung injury (ALI) in mice. Zarbock, A., Allegretti, M., Ley, K. Br. J. Pharmacol. 2008
Sialyltransferase ST3Gal-IV controls CXCR2-mediated firm leukocyte arrest during inflammation. Frommhold, D., Ludwig, A., Bixel, M.G., Zarbock, A., Babushkina, I., Weissinger, M., Cauwenbergs, S., Ellies, L.G., Marth, J.D., Beck-Sickinger, A.G., Sixt, M., Lange-Sperandio, B., Zernecke, A., Brandt, E., Weber, C., Vestweber, D., Ley, K., and Sperandio, M. J. Exp. Med 2008
Interleukin-17A inhibits the expansion of IL-17A-producing T cells in mice through "short-loop" inhibition via IL-17 receptor. Smith, E., Stark, M.A., Zarbock, A., Burcin, T.L., Bruce, A., Vaswani, D. Foley, P., Ley, K. J. of Immunology 2008
Upregulation of aldose reductase during foam cell formation as possible link between diabetes, hyperlipidemia and atherosclerosis. Gleissner, C., Sanders, J.M., Nadler, J.L., Ley, K. ATVB 2008
Blockade of CD40L signaling attenuates leukocyte accumulation and neointima formation after carotid denudation injury in apolipoprotein E-deficient mice. Li G, Sanders JM, Bevard MH, Chumley JW, Galkina EV, Ley K, Sarembock IJ. Am.J.Pathol. 2008
Neutrophil p21-activated kinase is critically involved in LPS-induced acute lung injury. Reutershan, J., Stockton, R., Zarbock, A., Sullivan, G.W., Chang, D., Scott, D., Schwartz, M.A., Ley, K. American Journal of Respiratory and Critical Care Medicine 2007
Interleukin-23 is required for neutrophil homeostasis in normal and neutrophilic mice. Smith, E., Zarbock, A., Stark, M.A., Burcin, T.L., Bruce, A.C., Foley, P., Ley, K. Journal of Immunology 2007
Initiation of Protein O-Glycosylation by Polypeptide GalNAcT-1 in Vascular Biology and Humoral Immunity Tenno, M., Ohtsubo, K., Hagen, F.K., Ditto, D., Zarbock, A., Schaerli, P., von Andrian, U.H., Ley, K., Le, D., Tabak, L.A., Marth, J.D. Mol. Cell. Biol. 2007
CXCR6 Promotes Atherosclerosis by Supporting T-Cell Homing, Interferon-{gamma} Production, and Macrophage Accumulation in the Aortic Wall. Galkina E, Harry BL, Ludwig A, Liehn EA, Sanders JM, Bruce A, Weber C, Ley K. Circulation 2007
Statistical identification of differentially labeled peptides from liquid chromatography tandem mass spectrometry. Cho H, Smalley DM, Theodorescu D, Ley K, Lee JK. Proteomics 2007
The Duffy antigen receptor for chemokines in acute renal failure: A facilitator of renal chemokine presentation. Zarbock A, Schmolke M, Bockhorn SG, Scharte M, Buschmann K, Ley K, Singbartl K. Crit Care Med. 2007
G{alpha}i2 is required for chemokine-induced neutrophil arrest. Zarbock A, Deem TL, Burcin TL, Ley K. Blood 2007
The Duffy antigen receptor for chemokines in acute renal failure: A facilitator of renal MD chemokine presentation. Zarbock, Alexander MD; Schmolke, Mirco PhD; Bockhorn, Susanne Groe PhD; Scharte, Marion MD; Buschmann, Kirsten MD; Ley, Klaus MD; Singbartl, Kai MD Crit Care Med. 2007
Therapeutic anti-inflammatory effects of myeloid cell adenosine receptor A2a stimulation in lipopolysaccharide-induced lung injury. Jörg Reutershan, Rebecca E. Cagnina, Daniel Chang, Joel Linden, and Klaus Ley J Immunol. 2007
Enhanced targeting of ultrasound contrast agents using acoustic radiation force. J. Rychak, A. Klibanov, K. Ley, J. Hossack Ultrasound Med Biol. 2007
Spleen Tyrosine Kinase Syk Is Necessary for E-Selectin-Induced AlphaLBeta2 Integrin-Mediated
Rolling on Intercellular Adhesion Molecule-1
Alexander Zarbock, Clifford A. Lowell, and Klaus Ley Immunity 2007
Induction of Vascular Permeability: betaPIX and GIT1 Scaffold the Activation of Extracellular Signal-regulated Kinase by PAK. Rebecca Stockton, Jörg Reutershan, David Scott, John Sanders, Klaus Ley, and Martin Alexander Schwartz Mol. Biol. Cell 2007
Blocking p21-activated Kinase Reduces Lipopolysaccharide-induced Acute Lung Injury by Preventing Polymorphonuclear Leukocyte Infiltration. Jörg Reutershan, Rebecca Stockton, Alexander Zarbock, Gail W. Sullivan, Daniel Chang, David Scott, Martin A. Schwartz, and Klaus Ley Am. J. Respir. Crit. Care Med. 2007
Dynamics of in silico leukocyte rolling, activation, and adhesion. Jonathan Tang, Klaus F Ley and C Anthony Hunt BMC Syst Biol. 2007
ENHANCED TARGETING OF ULTRASOUND CONTRAST AGENTS USING ACOUSTIC RADIATION FORCE.  J. Rychak, A. Klibanov, K. Ley, J. Hossack Ultrasound in Medicine & Biology 2007
Induction of Dendritic Cell-like Phenotype in Macrophages during Foam Cell Formation. HyungJun Cho, Pavel Shashkin, Christian Albert Gleissner, Dane Morris Dunson, Nitin Jain, Jae Lee, Yury Miller, and Klaus Ley Physiol. Genomics 2007
Proteomic discovery of 21 proteins expressed in human plasma-derived but not platelet-derived microparticles David M. Smalley, Karen E. Root, HyungJun Cho, Mark M. Ross, Klaus Ley Thrombosis and Haemostasis 2007
2,3-Sialyltransferase-IV is essential for L-selectin ligand function in inflammation Markus Sperandio, M.D., David Frommhold, Inna Babushkina, Lesley G. Ellies, Timothy S. Olson, Michael L. Smith, Benedikt Fritzsching, Eva Pauly, David F. Smith, Rainer Nobiling, Otwin Linderkamp, Jamey D. Marth, Klaus Ley European Journal of Immunology 2006
Complete reversal of acid-induced acute lung injury by blocking of platelet-neutrophil aggregation Alexander Zarbock, Kai Singbartl and Klaus Ley J. Clin. Invest. 2006
Leukocyte phosphoinositide-3 kinase {gamma} is required for chemokine-induced, sustained adhesion under flow in vivo David F. Smith, Tracy L. Deem, Anthony C. Bruce, Jörg Reutershan, Daniel Wu and Klaus Ley Journal of Leukocyte Biology 2006
Targeted ultrasound contrast agent for molecular imaging of inflammation in high-shear flow A. L. Klibanov , J. J. Rychak, W. C. Yang, S. Alikhani, B. Li, S. Acton, J. R. Lindner, K. Ley, S. Kaul Contrast Media & Molecular Imaging 2006
Antibody Blockade of CCL25/CCR9 Ameliorates Early but not Late Chronic Murine Ileitis. J. Rivera–Nieves, J. Ho, G. Bamias, N. Ivashkina, K. Ley, M. Oppermann, F. Cominelli Gastroenterology 2006
Platelet-neutrophil-interactions: Linking hemostasis and inflammation.  A. Zarbock, R. Polanowska-Grabowska, K. Ley Blood Reviews 2006
Sphingosine-1 Phosphate Prevents Monocyte/Endothelial Interactions in Type 1 Diabetic NOD Mice Through Activation of the S1P1 Receptor Angela M. Whetzel, David T. Bolick, Suseela Srinivasan, Timothy L. Macdonald, Margaret A. Morris, Klaus Ley, Catherine C. Hedrick Circulation Research 2006
Deformable gas-filled microbubbles targeted to P-selectin Joshua J. Rychak, Jonathan R. Lindner, Klaus Ley, and Alexander L. Klibanov Journal of Controlled Release 2006
Selectin ligands promote ultrasound contrast agent adhesion under shear flow. J. J. Rychak, B. Li, S. T. Acton, A. Leppänen, R. D. Cummings, K. Ley, and A. L. Klibanov Mol. Pharmaceutics 2006
Hold on to Your Endothelium: Postarrest Steps of the Leukocyte Adhesion Cascade Klaus Ley and Alexander Zarbock Immunity 2006
Lymphocyte recruitment into the aortic wall before and during development of atherosclerosis is partially L-selectin dependent. Galkina, E., Kadl, A., Sanders, J., Varughese, D., Sarembock, I.J., Ley K. J. Exp. Med. 2006
ICAM-1 and LFA-1 play critical roles in LPS-induced neutrophil recruitment into alveolar space. Basit A, Reutershan J, Morris MA, Ley K.   Am.J. Physiol: Lung Cell. Mol. Physiol 2006
Critical role of endothelial CXCR2 in LPS-induced neutrophil migration into the lung. Reutershan, J., Morris, M.A. Burcin, T.L. Smith, D.F., Chang, D. Saprito, M.S., Ley, K. J. Clin. Invest. 2006
Critical role of endothelial PSGL-1 in chronic murine ileitis. Rivera-Nieves, J., Burcin, T., Olson, T.S., Morris, M.A., McDuffie, M., Cominelli, F., Ley, K. J. Exp. Med. 2006
The primary defect in experimental ileitis originates from a non-hematopoietic source. Olson TS, Morris MA, Scott KGE, Reuter BK, Brucin TL, Hancock LN, Meddings JB, Cominelli F, Ley K, Pizarro TT J. Exp. Med. 2006
Protective Effects of Isoflurane Pretreatment in Endotoxin-induced Lung Injury. Reutershan, J., Hayes, J., Ley, K. Anesthesiology 2006
Targeting Mucosal Addressin Cellular Adhesion Molecule (MAdCAM)-1 to Non-Invasively Image Experimental Crohn's Disease. Bachmann, C., Klibanov, A.L., Olson, T.S., Cominelli, F., Ley, K., Lindner, J.R., Pizarro, T.T. Gastroenterology 2006
Induction of LFA-1-dependent neutrophil rolling on ICAM-1 by engagement of E-selectin. Chesnutt, B.C., Smith, D.F., Raffler, N.A., Smith, M.L., White, E.J., Ley, K. Microcirculation 2006
Protection from ileitis maps to PPAR? in mice. Sugawara K, Olson T, Moskaluk CA, Stevens B, Hoang S, Kozaiwa K, Cominelli F, Ley K, McDuffie M. Gastroenterology 2005
Preferential migration of effector CD8+ T-cells into the interstitium of the normal lung. Galkina, E., Thatte, J., Dabak, V., Williams, M.B., Ley, K., Braciale, T.J. J. Clin. Invest. 2005
Transient T cell accumulation in lymph nodes and sustained lymphopenia in mice treated with FTY720. Morris, M.A., Gibb D.R., Brinkmann, V., Straume, M., Ley, K. Eur. J. Immunol. 2005
Arterial Macrophages and Regenerating Endothelial Cells Express P-selectin in Atherosclerosis-Prone Apolipoprotein E-Deficient Mice. Li, G., Sanders, J.M., Phan, E.T., Ley, K., Sarembock, I.J. Am J. Pathol. 2005
Sphingosine kinase 2 is required for modulation of lymphocyte traffic by FTY720. Kharel, Y.,  Lee, S., Snyder, A.H., Sheasley-O’Neill, S.L., Morris, M.A., Setiady, Y., Zhu, R., Zigler, M.A., Burcin, T.L., Ley, K., Tung, K.S.K., Engelhard, V.H., Macdonald, T.L., Lynch, K.R. J. Biol. Chem. 2005
The Platelet Microparticle Proteome. Garcia, B.A., Smalley, D.M., Cho, H., Shabanowitz, J., Ley, K., Hunt, D.F. J Proteome Res. 2005
Linkage to peroxisome proliferator-activated receptor-gamma in SAMP1/YitFc mice and in human Crohn's disease. Sugawara K, Olson T, Moskaluk CA, Stevens B, Hoang S, Kozaiwa K, Cominelli F, Ley K, McDuffie M. Gastroenterology 2005
Sequential recruitment of neutrophils into lung and bronchoalveolar lavage fluid in LPS-induced acute lung injury. Reutershan J, Basit A, Galkina EV, Ley K. Am J Physiol Lung Cell Mol Physiol. 2005
GRO Family Chemokines are Specialized for Monocyte Arrest from Flow. Smith DF, Galkina E, Ley K, Huo Y. Am J Physiol Heart Circ Physiol. 2005
Phagocytosis of apoptotic neutrophils regulates granulopoiesis via IL-23 and IL-17. Stark MA, Huo Y, Burcin TL, Morris MA, Olson TS, Ley K. Immunity 2005
L-selectin, alpha 4 beta 1, and alpha 4 beta 7 integrins participate in CD4+ T cell recruitment to chronically inflamed small intestine. Rivera-Nieves J, Olson T, Bamias G, Bruce A, Solga M, Knight RF, Hoang S, Cominelli F, Ley K. J Immunol. 2005
CXCR2- and E-Selectin-induced Neutrophil Arrest during Inflammation In Vivo.
Smith ML, Olson TS, Ley K. J Exp Med. 2004
Critical role of macrophage 12/15-lipoxygenase for atherosclerosis in apolipoprotein E-deficient mice.
Huo Y, Zhao L, Hyman MC, Shashkin P, Harry BL, Burcin T, Forlow SB, Stark MA, Smith DF, Clarke S, Srinivasan S, Hedrick CC, Pratico D, Witztum JL, Nadler JL, Funk CD, Ley K. Circulation 2004
A novel method to analyze leukocyte rolling behavior in vivo.
Dunne JL, Goobic AP, Acton ST, Ley K. Biol. Proced. Online 2004
Expanded B cell population blocks regulatory T cells and exacerbates ileitis in a murine model of Crohn disease.
Olson TS, Bamias G, Naganuma M, Rivera-Nieves J, Burcin TL, Ross W, Morris MA, Pizarro TT, Ernst PB, Cominelli F, Ley K. J. Clin. Invest. 2004
Binding and detachment dynamics of microbubbles targeted to P-selectin under controlled shear flow.
Takalkar AM, Klibanov AL, Rychak JJ, Lindner JR, Ley K. J. Control Release 2004
Autoperfused mouse flow chamber reveals synergistic neutrophil accumulation through P-selectin and E-selectin.
Smith ML, Sperandio M, Galkina EV, Ley K. J. Leukoc. Biol. 2004
Targeted Disruption of cd73/Ecto-5'-Nucleotidase Alters Thromboregulation and Augments Vascular Inflammatory Response. Patrycja Koszalka, Burcin Özüyaman, Yuqing Huo, Alma Zernecke, Ulrich Flögel, Norbert Braun, Anja Buchheiser, Ulrich K.M. Decking, Michael L. Smith, Jean Sévigny, Adrian Gear, Artur-Aron Weber, Andrei Molojavyi, Zhaoping Ding, Christian Weber, Klaus Ley, Herbert Zimmermann, Axel Gödecke, Jürgen Schrader Circ. Res.


Vav GEFs are required for beta2 integrin-dependent functions of neutrophils.

M. Angelica Martinez Gakidis, Xavier Cullere, Timothy Olson, Julie L. Wilsbacher, Bin Zhang, Sheri L. Moores, Klaus Ley, Wojciech Swat, Tanya Mayadas, and Joan S. Brugge

Eur. J. Cell Biol. 2004
Microviscometry reveals reduced blood viscosity and altered shear rate and shear stress profiles in microvessels after hemodilution. David S. Long, Michael L. Smith, Axel R. Pries, Klaus Ley, and Edward R. Damiano Proc Natl Acad Sci 2004
Alpha4beta1 integrin (VLA-4) blockade attenuates both early and late leukocyte recruitment and neointimal growth following carotid injury in apolipoprotein E (-/-) mice. Kurt G. Barringhaus, J. William Phillips, Jayant S. Thatte,
John M. Sanders, Ann C. Czarnik, Daniel K. Bennett, Klaus F. Ley,
Ian J. Sarembock
J. Vasc. Res. 2004
Critical role of platelet P-selectin in the response to arterial injury in apolipoprotein-E-deficient mice. David Manka; S. Bradley Forlow; John M. Sanders; Debra Hurwitz; Daniel K. Bennett; Samuel A. Green; Klaus Ley; Ian J. Sarembock Arterioscler. Thromb. Vasc. Biol. 2004
Autoperfused mouse flow chamber reveals synergistic neutrophil accumulation through P-selectin and E-selectin. Michael L. Smith, Markus Sperandio, Elena V. Galkina and Klaus Ley J Leukoc Biol 2004
Rosiglitazone reduces the accelerated neointima formation after arterial injury in a mouse injury model of type 2 diabetes. J. William Phillips, MD; Kurt G. Barringhaus, MD; John M. Sanders, BS; Zandong Yang, MD; Meng Chen, MD; Sean Hesselbacher, BS; Ann C. Czarnik, BS; Klaus Ley, MD; Jerry Nadler, MD; Ian J. Sarembock, MB, ChB, MD Circulation 2003
Circulating activated platelets exacerbate atherosclerosis in apolipoprotein E deficient mice. Huo, Y., Schober, A., Forlow, S.B., Smith, D.F., Hyman, M.C., Jung, S., Littman, D.R., Weber, C., Ley, K. Nature Medicine 2003
Expression of CXCL16 in human T cells (letter to the editor). Shashkin, P., Simpson, D., Chesnutt, B., Ley, K. Arteriosclerosis, Thromb. and Vasc. Biol. 2003
LFA-1 is required for retention of effector CD8 T cells in mouse lungs. Thatte, J., Dabak, V., Williams, M.B., Braciale, T.J., Ley, K. Blood 2003
P-selectin glycoprotein ligand-1 mediates L-selectin dependent rolling in venules. Sperandio, M., Smith, M.L., Forlow, S.B., Olson, T.S., Xia, L., McEver, R.P., Ley, K. J.Exp. Med. 2003
Single injection of P-selectin or PSGL-1 monoclonal antibody blocks neointima formation after arterial injury in apolipoprotein-E deficient mice. Phillips JW, Barringhaus KG Sanders JM, Hesselbacher SE, Czarnik AC, Manka D, Vestweber D, Ley K, Sarembock IJ. Circulation 2003
Local-pooled-error test for identifying differentially expressed genes with a small number of replicated microarrays. Nitin Jain, Jayant Thatte, Thomas Braciale, Klaus Ley, Michael O’Connell and Jae K. Lee Bioinformatics 2003
Vascular protection by estrogen in ischemia-reperfusion injury requires endothelial nitric oxide synthase. Prorock, A.J., Hafezi-Moghadam, A., Laubach, V.E., Liao, J., Ley, K. Am. J. Physiol. 2003
Mac-1, but Not LFA-1, Uses Intercellular Adhesion Molecule-1 to Mediate Slow Leukocyte Rolling in TNF-{alpha}-Induced Inflammation. Jessica L. Dunne, Robert G. Collins, Arthur L. Beaudet, Christie M. Ballantyne, and Klaus Ley J Immunol 2003
Acute cardiovascular protective effects of corticosteroids are mediated by non-transcriptional activation of endothelial nitric oxide synthase. Hafezi-Moghadam, A., Simoncini, T., Yang, Z., Limbourg, F.P., Plumier, J.-C., Rebsamen, M.C., Hsieh, C.M., Chui, D.S., Thomas, K.L., Prorock, A.J., Laubach, V.E., Moskowitz, M.A., French, B.A., Ley, K., Liao, J.K. Nature Medicine 2002
Automatic tracking of rolling leukocytes in vivo. Acton, S.T., Wethmar, K., Ley, K. Microvascular Research 2002
Control of leukocyte rolling velocity in TNF-alpha-induced inflammation by LFA-1 and Mac-1. Dunne, J.L., Ballantyne, C.M., Beaudet, A.L., Ley, K. Blood 2002
Deposition of platelet RANTES triggering monocyte recruitment requires P-selectin and is involved in neointima formation after arterial injury. Schober, A., Manka, D., von Hundelshausen, P., Huo, Y; Hanrath, P., Sarembock, I.J., Ley, K., Weber, C. Circulation 2002
L-selectin is required for fMLP- but not C5a-induced sequestration of neutrophils in the pulmonary circulation. Olson, T.S., Singbartl, K., Ley, K. Am. J. Physiol.: Regulatory, Integrative and Comparative Physiology 2002
P-selectin Glycoprotein Ligand-1-deficient mice have impaired leukocyte tethering to E-selectin under flow. Xia, L., Sperandio, M., Yago, T., McDaniel, J.M., Cummings, R.D., Pearson-White, S., Ley, K., McEver, R.P. J. Clin. Invest. 2002
Severely reduced neutrophil adhesion and impaired host defense against fecal and commensal bacteria in CD18-/-P-selectin-/- double null mice. Forlow, S.B., Foley, P.L., Ley, K. FASEB J. 2002
Sialyltransferase specificity in selectin ligand formation. Ellies, L.G., Sperandio, M., Underhill, G.H., Yousef, J., Smith, M.L., Priatel, J.J., Kansas, G.S., Ley, K., Marth, J.D. Blood 2002
T cell requirement for the development of chronic ulcerative dermatitis in E- and P-selectin deficient mice. Forlow, S.B., White, E.J., Thomas, K., Bagby, G.J., Foley, P.L., Ley, K. J. Immunol. 2002
Tracking leukocytes in vivo with shape and size constrained active contours. Ray, N., Acton, S.T., Ley, K. IEEE Transactions on Medical Imaging 2002
Viscosity-independent velocity of neutrophils rolling on P-selectin in vitro or in vivo. Smith, M.L., Smith, M.J., Lawrence, M.B., Ley, K. Microcirculation 2002
Binding of function-blocking mAbs to mouse and human P-selectin glycoprotein ligand-1 peptides with and without tyrosine sulfation. Aravinda Thatte, Scott Ficarro, Karen R. Snapp, Martin K. Wild, Dietmar Vestweber, Donald F. Hunt, and Klaus F. Ley J Leukoc Biol 2002
A CD2-GFP Transgenic mouse reveals VLA-4-dependent CD8+ Lymphocyte Rolling in the Inflamed Microcirculation. Singbartl, K., Thatte, J., Smith, M.L., Day, K., Ley, K. J. Immunol. 2001
Dermal and pulmonary inflammatory disease in E/P-selectin double-null mice is reduced in triple-selectin-null mice. Collins, R.G., Jung, U., Ramirez, M., Bullard, D.C., Hicks, J., Smith, C.W., Ley, K., Beaudet, A.L. Blood 2001
Differential requirements for core2 glucosaminyltransferase for endothelial L-selectin ligand function in vivo. Sperandio, M., Forlow, S.B., Thatte, J., Ellies, L.G., Marth, J.D., Ley, K. J. Immunol. 2001
Essential role of neutrophils in germ cell-specific apoptosis following ischemia/reperfusion injury of the mouse testis. Lysiak, J.L., Turner, S.D., Nguyen, Q.A., Singbartl, K., Ley, K., Turner, T.T. Biology of Reproduction 2001
Increased granulopoiesis through interleukin-17 and granulocyte colony-stimulating factor in leukocyte adhesion molecule-deficient mice. Forlow, S.B., Schurr, J.R., Kolls, J.K., Bagby, G.J., Schwarzenberger, P.S., Ley, K. Blood 2001
L-selectin shedding regulates leukocyte recruitment. Hafezi-Moghadam, A., Thomas, K.L., Prorock, A.J., Huo, Y., Ley, K. J.Exp.Med. 2001
Platelet, not endothelial P-selectin is critical for neutrophil-mediated acute postischemic renal failure. Singbartl, K., Forlow, S.B., Ley, K. FASEB J. 2001
RANTES deposition by platelets triggers monocyte arrest on inflamed and atherosclerotic endothelium. von Hundelshausen, P., Weber, K.S.C., Huo, Y., Proudfoot, A.E.I., Nelson, P., Ley, K., Weber, C. Circulation 2001
Selectin-independent leukocyte rolling and adhesion in mice deficient in E-, P-, and L-selectin and ICAM-1. Forlow, S.B., Ley, K. Am. J. Physiol. 2001
Severe impairment of leukocyte rolling in core 2 glucosaminyltransferase deficient mice. Sperandio, M., Thatte, A., Foy, D., Ellies, L., Marth, J.D., Ley, K. Blood 2001
The chemokine KC, but not MCP-1 triggers arrest of monocytes in carotid arteries with early atherosclerotic lesions. Huo, Y., Forlow, S.B., Weber, C., Jung, S. Littman, D.R., Ley, K. J. Clin. Invest. 2001
Ultrasound assessment of inflammation and renal tissue injury with microbubbles targeted to P-selectin. Lindner JR, Song J, Christiansen J, Klibanov AL, Xu F, Ley K. Circulation 2001
Antibody blockade of ICAM-1 and VCAM-1 ameliorates inflammation in the SAMP-1/Yit adoptive transfer model of crohn's disease in mice.  R. Burns, J. Rivera-Nieves, C. Moskaluk, S. Matsumoto, F. Cominelli, K. Ley Gastroenterology 2001
Absence of P-selectin but not ICAM-1 limits neointimal growth after arterial injury in apoliprotein-E (apoE)-deficient mice. Manka, D., Collins, R., Ley, K., Beaudet, A.L., Sarembock, I.J. Circulation 2000
Blocking P-selectin protects from ischemia-reperfusion induced severe acute renal failure. Singbartl, K., Green, S.A., Ley, K. FASEB J. 2000
Delayed onset of inflammation in protease-activated receptor-2 (PAR-2) deficient mice. Lindner, J.R., Kahn, M.L., Coughlin, S.R., Sambrano, G.R., Schauble, E., Bernstein, D., Foy, D., Hafezi-Moghadam, A., Ley, K. J. Immunol. 2000
Intercellular Adhesion Molecule-1 (ICAM-1) is required for chemoattractant-induced leukocyte adhesion in resting, but not inflamed venules in vivo. Foy, D.S., Ley, K. Microvascular Research 2000
Leukocyte arrest during cytokine-dependent inflammation in vivo Kunkel, E.J., Dunne, J.L., Ley, K. J. Immunol 2000
Microbubble persistence in the microcirculation during ischemia-reperfusion and inflammation: integrin- and complement-mediated adherence to activated leukocytes. Lindner, J.R., Coggins, M.P., Kaul, S., Klibanov, A.L., Brandenburger, G.H., Ley, K. Circulation 101 2000
Noninvasive imaging of inflammation by ultrasound detection of phagocytosed microbubbles. Lindner, J.R., Dayton, P.A., Coggins, M.P., Ley, K., Song, J., Ferrara, K., Kaul, S. Circulation 2000
Noninvasive in vivo magnetic resonance imaging of injury-induced neointima formation in the carotid artery of the apolipoprotein-E null mouse. Manka, D., Gilson, W., Sarembock, I.J., Ley, K., Berr, S.S. J. Magnetic Resonance Imaging 2000
Noninvasive ultrasound imaging of inflammation using microbubbles targeted to activated leukocytes. Lindner, J.R., Song, J., Xu, F., Klibanov, A.L., Singbartl, K., Ley, K., Kaul, S. Circulation 2000
Protection from ischemia-reperfusion induced severe renal failure by blocking E-selectin. Singbartl, K, Ley, K. Critical Care Medicine 2000
Role of Vascular Cell Adhesion Molecule-1 (VCAM-1) and fibronectin connecting segment-1 in monocyte rolling and adhesion on early atherosclerotic lesions. Huo, Y., Hafezi-Moghadam, A., Ley, K. Circ. Res. 2000
Severe inflammatory defect and reduced viability in CD18 and E-selectin double mutant mice. Forlow, S.B., White, E.J., Barlow, S.C., Feldman, S.H., Lu, H., Bagby, G.J., Beaudet, A.L., Bullard, D.C., Ley, K. J. Clin. Invest. 2000
The CD24/P-selectin binding pathway initiates lung arrest of human A125 adenocarcinoma cells. Friederichs, J., Zeller, Y., Hafezi-Moghadam, A., Mecke, C., Gröne, H.-J., Ley, K., Altevogt, P. Cancer Research 2000
Vascular protective effects of oestrogen mediated by phosphatidylinositol 3-OH kinase. Simoncini, T., Hafezi-Moghadam, A., Brazil, D., Ley, K., Chin, W.W., Liao, J.K. Nature 2000


Review Articles in Peer-Reviewed Journals

Publication Year
Getting to the site of inflammation: the leukocyte adhesion cascade updated. Klaus Ley, Carlo Laudanna, Myron I. Cybulsky& Sussan Nourshargh Nat Rev Immunol. 2007
Vascular Adhesion Molecules in Atherosclerosis. Galkina E, Ley K. Arterioscler Thromb Vasc Biol. 2007
Platelet-neutrophil-interactions: Linking hemostasis and inflammation Alexander Zarbock, Renata K. Polanowska-Grabowska and Klaus Ley Blood Reviews 2007
Effects of Native and Modified Low-Density Lipoproteins on Monocyte Recruitment in Atherosclerosis. Gleissner CA, Leitiniger N, Ley K. Hypertension 2007
Targeted ultrasound contrast agent for molecular imaging of inflammation in high-shear flow A. L. Klibanov , J. J. Rychak, W. C. Yang, S. Alikhani, B. Li, S. Acton, J. R. Lindner, K. Ley, S. Kaul Contrast Media & Molecular Imaging 2006
Analysis of Leukocyte Rolling In Vivo and In Vitro Markus Sperandio, John Pickard, Sunil Unnikrishnan, Scott T. Acton and Klaus Ley Methods in Enzymology 2006
Neutrophil-Regulatory Tn Lymphocytes Ley K, Smith E, Stark MA. Immunol Res. 2006
Leukocyte recruitment and vascular injury in diabetic nephropathy. Elena Galkina and Klaus Ley J Am Soc Nephrol 2006
Macrophage differentiation to foam cells. Shashkin P, Dragulev B, Ley K. Curr Pharm Des. 2005
Bench-to-bedside review: acute respiratory distress syndrome - how neutrophils migrate into the lung. Reutershan J, Ley K. Crit Care 2005
L-selectin: mechanisms and physiological significance of ectodomain cleavage. Smalley DM, Ley K. J Cell Mol Med. 2005
The physiology and pathophysiology of P-selectin. Sperandio M., Ley K. Modern Aspects in Immunobiology 2005
L-selectin in inflammation, infection and immunity Nikolai A. Raffler, Jesús Rivera-Nieves and Klaus Ley Drug Discovery Today: Therapeutic Strategies 2005
Role of platelets in the development of atherosclerosis.
Huo Y, Ley KF. Trends Cardiovasc. Med. 2004
Leukocyte recruitment and acute renal failure.
Singbartl K, Ley K. J. Mol. Med. 2004 2004
Trafficking of natural killer cells.
Morris MA, Ley K. Curr. Mol. Med. 2004
Selectins in T-cell recruitment to non-lymphoid tissues and sites of inflammation.
Ley K, Kansas GS. Nat. Rev. Immunol. 2004
Cerebral vasospasm after subarachnoid hemorrhage: putative role of inflammation. Dumont AS, Dumont RJ, Chow MM, Lin CL, Calisaneller T, Ley KF, Kassell NF, Lee KS. Neurosurgery 2003
The role of selectins in inflammation and disease Klaus Ley Trends in Molecular Medicine 2003
The role of inflammation in vascular injury and repair. Davis C, Fischer J, Ley K, Sarembock IJ. J Thromb Haemost. 2003
Arrest Chemokines Klaus Ley Microcirculation 2003
Integration of inflammatory signals by rolling neutrophils Klaus Ley Immunological Reviews 2002
Chemokines and chemokine receptors in leukocyte trafficking Timothy S. Olson and Klaus Ley Am J Physiol Regul Integr Comp Physiol 2002
Pathways and Bottlenecks in the Web of Inflammatory Adhesion Molecules and Chemoattractants Ley K. Immunologic Research 2001
Adhesion molecules and atherogenesis. Y. Huo and K. Ley Acta Physiol. Scand. 2001

Editorials, Commentaries, Forewords
Publication Year
To transmigrate or not to transmigrate. Klaus Ley Blood 2007
Hold on to your endothelium: postarrest steps of the leukocyte adhesion cascade. Ley K, Zarbock A. Immunity 2006
Double jeopardy: how soluble P-selectin activates leukocytes in peripheral arterial occlusive disease. Galkina E, Ley K. Circ Res. 2006
Signals for lymphocyte egress. Ley K, Morris M. Nat Immunol 2005
Oxidative modification of leukocyte adhesion. Ley K, Deem TL. Immunity 2005
Weird and weirder: how circulating chemokines coax neutrophils to the lung.
Ley K. Am. J. Physiol. Lung Cell Mol. Physiol. 2004
Sulfated sugars for rolling lymphocytes. Klaus Ley J Exp Med 2003
Healing without inflammation? Klaus Ley Am J Physiol Regul Integr Comp Physiol


Arrest, Migration, Survival and Proliferation of Leukocytes and Vascular Cells: The Many Faces of Chemokine Biology Klaus Ley Microcirculation 2003
Plugging the leaks Klaus Ley Nature Medicine 2001
Adhesion molecules in lymphocyte trafficking and colitis. Ley K, Burns C. Gastroenterology 2001
VCAM-1 is critical in atherosclerosis Klaus Ley and Yuqing Huo J. Clin. Invest. 2001



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